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M94A2751.TXT
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1994-10-25
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Document 2751
DOCN M94A2751
TI NASBA HIV-1 RNA amplification compared to HIV-1 RNA-PCR on plasma or
serum samples: a Belgian field evaluation.
DT 9412
AU Vandamme AM; Van Dooren S; Kok W; Goubau P; Fransen K; Kievits T;
Desmyter J; Rega Institute, Leuven-Belgium.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):233 (abstract no. PB0363). Unique
Identifier : AIDSLINE ICA10/94369824
AB The presence of HIV-1 RNA in the plasma or serum of European and African
patients was monitored using RNA-PCR and the new HIV-1 NASBA system
involving an isothermal amplification. Identical RNA extraction
procedures, provided by the NASBA system, were used for both methods.
Both NASBA and RNA-PCR are more sensitive than p24 for the detection of
HIV-1 free virus in blood: 18 of the 35 p24 tested seropositives were
p24 negative while only 2 were negative for both NASBA and RNA-PCR
(Table). The detection limit for HIV-1 RNA was slightly better for
NASBA: in 8 microliters plasma or serum, 0.01 CCID50 of spiked HIV-IIIB
was reported positive for NASBA but indeterminate (ID) for RNA-PCR. Two
seronegative samples were NASBA and RNA-PCR positive or indeterminate.
This was probably due to a sample carryover contamination, which can be
avoided by more careful lab practises. There was no cross reactivity
with HIV-2 or HTLV-I. The extraction method used allowed us to detect
HIV-1 RNA equally well in plasma on heparine or on EDTA. TABULAR DATA,
SEE ABSTRACT VOLUME.
DE Belgium Comparative Study Gene Amplification/*GENETICS Human HIV
Core Protein p24/BLOOD HIV Infections/*DIAGNOSIS/MICROBIOLOGY HIV
Seronegativity HIV Seropositivity/DIAGNOSIS/MICROBIOLOGY
HIV-1/*GENETICS Polymerase Chain Reaction/*METHODS Predictive Value of
Tests RNA, Viral/*BLOOD/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).